Cy3 Streptavidin

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Other Names
SAv-Cy3
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405215 100 µg 80€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Cy3 is useful for detecting biotinylated antibodies. The excitation of Cy3 by 550 nm laser light induces a light emission maximum of 570 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with Cy3 under optimal conditions. The solution is free of unconjugated Cy3.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-Cy3 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF

Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cy3 conjugation.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Application Notes

The average molecular weight of Streptavidin-Cy3 is 60 kD and Streptavidin alone is 52 kD.

Application References
  1. Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed
  2. Thoma CR, et al. 2013. J Biomol Screen. 18:1330. PubMed
  3. Sunshine J, et al. 2014. J. Virol. 88:1354. PubMed
  4. Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed
  5. Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
Product Citations
  1. Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed
  2. Kelm W 2013. J Biomol Screen. 18:1330. PubMed
  3. Sunshine J, et al. 2014. J Virol. 88:1354. PubMed
  4. Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed
  5. Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
  6. Fukuyama T, et al. 2015. J Pharmacol Exp Ther. 354: 394 - 405. PubMed
  7. Sharma N, et al. 2015. J Immunol. 195: 5227 - 5236. PubMed
  8. Atreya I, et al. 2016. J Crohns Colitis. 10: 1132 - 1143. PubMed
  9. Okamura Y, et al. 2017. Allergol Int. 10.1016/j.alit.2017.03.002. PubMed
  10. He W, et al. 2018. Immunity. 49:1175. PubMed
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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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