Brilliant Violet 650™ Streptavidin

Pricing & Availability
Regulatory Status
Other Names
SAv-Brilliant Violet 650™
Ave. Rating
Submit a Review
Product Citations
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 650™.
  • SAV_BV650_043012
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 650™.
See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
405231 100 µL 185€
Check Availability

Need larger quantities of this item?
Request Bulk Quote
405232 100 µg 370€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 650™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 650™ by 405 nm laser light induces a fluorescence maximum emission of 645 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Cat. No. 405231: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Cat. No. 405232: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Streptavidin was conjugated with Brilliant Violet 650™ under optimal conditions.
Cat. No. 405231: 0.1 mg/ml
Cat. No. 405232: 0.5 mg/ml
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 650™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 650™ conjugation. For immunofluorescent staining, we recommend using ≤0.125 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 650™ is 340 kD and Streptavidin alone is 52 kD.

Product Citations
  1. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  2. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  3. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  4. Williams B, et al. 2015. Dis Model Mech. 8: 805-815. PubMed
  5. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  6. Guo C, et al. 2021. Cell Rep Med. 2:100448. PubMed
  7. Kitajima Y, et al. 2022. Cells. 11:. PubMed
  8. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  9. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  10. Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed
  11. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  12. Yamamoto T, et al. 2015. Sci Transl Med. 7: 298ra120. PubMed
  13. Turner JS, et al. 2021. Nature. 595:421. PubMed
  14. Martin S, et al. 2021. iScience. 24:103185. PubMed
  15. Wells DK, et al. 2020. Cell. 183:818. PubMed
  16. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  17. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  18. Laczkó D, et al. 2020. Immunity. 53:724. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account