- Clone
- HI30 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N816
- Other Names
- LCA, T200
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood lymphocytes were stained with CD45 (clone HI30) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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304043 | 25 tests | 168€ | ||||
304044 | 100 tests | 321€ |
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding. - Application References
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- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Kishihara K, et al. 1993. Cell 74:143.
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
- Nagano M, et al. 2007. Blood 110:151.
- Jiang Q, et al. 2008. Blood 112:2858. PubMed
- Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
- Lee J, et al. 2015. J Exp Med. 212:385. PubMed
- Breton G, et al. 2015. J Exp Med. 212:401. PubMed
- Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_2562498 (BioLegend Cat. No. 304043)
AB_2563812 (BioLegend Cat. No. 304044)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
- Distribution
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Hematopoietic cells, not expressed in circulating erythrocytes or platelets
- Function
- TCR and BCR mediated activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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- Thomas M. 1989. Annu. Rev. Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45
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Biotin anti-human CD45
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FITC anti-human CD45
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PE anti-human CD45
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PE/Cyanine5 anti-human CD45
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Purified anti-human CD45
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APC/Cyanine7 anti-human CD45
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PE/Cyanine7 anti-human CD45
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Alexa Fluor® 488 anti-human CD45
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Alexa Fluor® 647 anti-human CD45
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Pacific Blue™ anti-human CD45
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Alexa Fluor® 700 anti-human CD45
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PerCP anti-human CD45
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PerCP/Cyanine5.5 anti-human CD45
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Brilliant Violet 421™ anti-human CD45
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Brilliant Violet 570™ anti-human CD45
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Brilliant Violet 510™ anti-human CD45
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Brilliant Violet 605™ anti-human CD45
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Brilliant Violet 650™ anti-human CD45
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Purified anti-human CD45 (Maxpar® Ready)
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Brilliant Violet 785™ anti-human CD45
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Brilliant Violet 711™ anti-human CD45
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PE/Dazzle™ 594 anti-human CD45
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Alexa Fluor® 594 anti-human CD45
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APC/Fire™ 750 anti-human CD45
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Pacific Blue™ anti-human CD45
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APC anti-human CD45
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PE/Cyanine7 anti-human CD45
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PE/Dazzle™ 594 anti-human CD45
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TotalSeq™-A0391 anti-human CD45
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TotalSeq™-B0391 anti-human CD45
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TotalSeq™-C0391 anti-human CD45
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PerCP/Cyanine5.5 anti-human CD45
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APC/Fire™ 750 anti-human CD45
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PE/Fire™ 640 anti-human CD45
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APC/Fire™ 810 anti-human CD45
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Spark YG™ 570 anti-human CD45
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PE/Fire™ 700 anti-human CD45
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FITC anti-human CD45
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PerCP anti-human CD45
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Alexa Fluor® 660 anti-human CD45 Antibody
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Spark Violet™ 538 anti-human CD45
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Spark YG™ 593 anti-human CD45
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GMP APC/Fire™ 750 anti-human CD45
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Spark Violet™ 538 anti-human CD45
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GMP APC anti-human CD45
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Spark UV™ 387 anti-human CD45
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GMP Pacific Blue™ anti-human CD45
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GMP PerCP anti-human CD45
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GMP FITC anti-human CD45
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PE anti-human CD45
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GMP PE/Dazzle™ 594 anti-human CD45
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GMP PerCP/Cyanine5.5 anti-human CD45
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Spark Blue™ 515 anti-human CD45
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TotalSeq™-D0391 anti-human CD45
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