- 29E.2A3 (See other available formats)
- Other Names
- Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
- Mouse IgG2b, κ
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- Product Citations
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CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Full length human PD-L1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™ and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen sections1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 329711). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329716) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.
Clone 29E.2A3 does not work in Western blot applications7.
- Application References
- Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
- Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
- Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
- Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
- Haile, S et al. 2013. J. Immunol. 191:2829.
- RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
- Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.
AB_2629613 (BioLegend Cat. No. 329739)
AB_2629614 (BioLegend Cat. No. 329740)
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- PD-1 (PDCD1)
- Cell Type
- B cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Dendritic cells
- Biology Area
- Immunology, Cancer Biomarkers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
- Gene ID
- 29126 View all products for this Gene ID
- View information about CD274 on UniProt.org
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