- 390 (See other available formats)
- Other Names
- PECAM-1, EndoCAM
- Rat IgG2a, κ
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- Product Citations
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CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.Product Details
- Antibody Type
- Host Species
- C3H/HeJ mouse hematopoietic progenitor cell line 3
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- µl size: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)µg size: 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).
- Application References
- Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
- DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
- Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
- Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
- Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
- Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
- McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
- Chacko AM, et al. 2012. PLoS One 7:e34958.
- Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
- Product Citations
AB_2562186 (BioLegend Cat. No. 102423)
AB_2650892 (BioLegend Cat. No. 102424)
- Ig superfamily, 130-140 kD
Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
- CD38, αV/β3 integrin
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.
- Gene ID
- 18613 View all products for this Gene ID
- View information about CD31 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
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