Brilliant Violet 421 anti-human CD3 Antibody anti-CD3

Pricing & Availability

Clone: SK7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with CD3 (clone SK7) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

Compare all formats See Brilliant Violet 421™ spectral data

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344833

25 tests

140€

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344834

100 tests

296€

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.

Application References

Kan EA, et al. 1983. J. Immunol. 131:536.
Wood GS, et al. 1985. Am. J. Pathol. 120:371.
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
Carbone A, et al. 1999. Blood 93:2319. (IHC)
Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
Rutjens E, et al. 2007. J. Immunol. 178:1702.
Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Product Citations

Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed
Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
Hsiue EH, et al. 2021. Science. 371:. PubMed
Mudd PA, et al. 2022. Cell. 185:603. PubMed
Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
Kosti P, et al. 2021. STAR Protocols. 2:100723. PubMed

RRID

AB_2565674 (BioLegend Cat. No. 344833)
AB_2565675 (BioLegend Cat. No. 344834)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

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Description	Clone	Applications
APC/Fire™ 750 anti-human CD3	SK7	FC
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FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Alexa Fluor® 488 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
APC/Cyanine7 anti-human CD3	SK7	FC
Alexa Fluor® 700 anti-human CD3	SK7	FC
Pacific Blue™ anti-human CD3	SK7	FC
Alexa Fluor® 647 anti-human CD3	SK7	FC
Brilliant Violet 510™ anti-human CD3	SK7	FC
Brilliant Violet 421™ anti-human CD3	SK7	FC
Brilliant Violet 605™ anti-human CD3	SK7	FC
Brilliant Violet 711™ anti-human CD3	SK7	FC
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Brilliant Violet 785™ anti-human CD3	SK7	FC
PE/Dazzle™ 594 anti-human CD3	SK7	FC
Brilliant Violet 750™ anti-human CD3	SK7	FC
TotalSeq™-A0049 anti-human CD3	SK7	PG
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
TotalSeq™-C0049 anti-human CD3	SK7	PG
Spark Blue™ 550 anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
TotalSeq™-B0049 anti-human CD3	SK7	PG
Alexa Fluor® 660 anti-human CD3	SK7	FC
APC/Fire™ 810 anti-human CD3	SK7	FC
Spark NIR™ 685 anti-human CD3	SK7	FC
PE/Fire™ 640 anti-human CD3	SK7	FC
PE/Fire™ 700 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
APC/Fire™ 750 anti-human CD3	SK7	FC
GMP FITC anti-human CD3	SK7	FC
PE/Cyanine5 anti-human CD3 Antibody	SK7	FC
GMP PE anti-human CD3	SK7	FC
GMP APC anti-human CD3	SK7	FC
GMP PerCP/Cyanine5.5 anti-human CD3	SK7	FC
Spark YG™ 593 anti-human CD3	SK7	FC
GMP PerCP anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/30/2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.