Biotin anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
1A8_Biotin_082808.jpg
C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
  • 1A8_Biotin_082808.jpg
    C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
Cat # Size Price Quantity Avail. Save
127603 50 µg 52€
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127604 500 µg 184€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Ryzhov S, et al. 2011. J Immunol. 187:6120. PubMed
  2. Yang L, et al. 2012. J Immunol. 189:3741. PubMed
  3. Blazek K, et al. 2015. J Exp Med. 212:845. PubMed
  4. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  5. Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed
  6. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  7. Tang Y, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G156 - G165. PubMed
  8. Popova T, et al. 2016. PLoS One. 11: 0163163. PubMed
  9. De Cock J, et al. 2016. Cancer Res . 76(23):6778-6784. PubMed
  10. Haffner-Luntzer M, et al. 2017. Eur J Med Res. 10.1186/s40001-017-0264-y. PubMed
  11. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  12. de Mingo Pulido Á 2018. Cancer cell. 33:60. PubMed
  13. Best SA 2018. Cell metabolism. 27:935. PubMed
  14. Li Q, et al. 2018. Immunity. 48:258. PubMed
  15. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  16. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  17. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  18. Sahakian E, et al. 2017. J Leukoc Biol. 102:475. PubMed
Publication Library
RRID
AB_1186105 (BioLegend Cat. No. 127603)
AB_1186108 (BioLegend Cat. No. 127604)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Reviews

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Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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