Biotin anti-mouse IFNAR-1 Antibody

Pricing & Availability
Clone
MAR1-5A3 (See other available formats)
Regulatory Status
RUO
Other Names
IFNAR1, IFN-αR1, IFN-α/β Receptor Subunit 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MAR1-5A3_Biotin_070808.jpg
C57BL/6 mouse splenocytes stained with biotinylated MAR1-5A3, followed by Sav-PE
  • MAR1-5A3_Biotin_070808.jpg
    C57BL/6 mouse splenocytes stained with biotinylated MAR1-5A3, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
127305 25 µg 67€
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127306 100 µg 155€
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Description

IFNAR-1, the type I IFN receptor subunit 1, is coexpressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs (IFN-α/β). Type I IFNs are a group of structurally and functionally related cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor, and autoimmune responses. Ligand binding to the IFN-α/β receptor complex leads to the tyrosine phosphorylation and activation of IFNAR-1-associated Tyk2 and IFNAR-2-associated Jak1 signal transductions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Exogenously expressed murine IFNAR1 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IP, WB, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, ELISA1, and blocking8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127322) (Endotoxin <0.01 EU/µg).

Application References
  1. Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
  2. Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
  3. Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
  4. Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
  5. Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
  6. Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
  7. Marshall HD, et al. 2011. J Virol. epub. PubMed
  8. Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
  9. Jones GW, et al. 2011. FASEB J. 25:409. (Block)
  10. Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed
Product Citations
  1. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
RRID
AB_1134250 (BioLegend Cat. No. 127305)
AB_1134249 (BioLegend Cat. No. 127306)

Antigen Details

Distribution

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

Ligand/Receptor
IFN-α/β
Antigen References

1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391.

Gene ID
15975 View all products for this Gene ID
UniProt
View information about IFNAR-1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IFNAR-1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse IFNAR-1 MAR1-5A3 FC, IP, WB, ELISA, Block
Biotin anti-mouse IFNAR-1 MAR1-5A3 FC, IP, WB, ELISA
PE anti-mouse IFNAR-1 MAR1-5A3 FC
APC anti-mouse IFNAR-1 MAR1-5A3 FC
Ultra-LEAF™ Purified anti-mouse IFNAR-1 MAR1-5A3 FC, IP, WB, ELISA, Block
PE/Cyanine7 anti-mouse IFNAR-1 MAR1-5A3 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse IFNAR-1

  • Biotin anti-mouse IFNAR-1

  • PE anti-mouse IFNAR-1

  • APC anti-mouse IFNAR-1

  • Ultra-LEAF™ Purified anti-mouse IFNAR-1

  • PE/Cyanine7 anti-mouse IFNAR-1

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