Loading...

Biotin anti-mouse CD49b (pan-NK cells) Antibody

Pricing & Availability
Clone
DX5 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2
Isotype
Rat IgM, κ
Ave. Rating
Submit a Review
Product Citations
publications
DX5_Biotin_090707
C57BL/6 mouse splenocytes stained with biotinylated DX5, followed by Sav-PE
  • DX5_Biotin_090707
    C57BL/6 mouse splenocytes stained with biotinylated DX5, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
108903 50 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
108904 500 µg 219€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-propagated NK1.1+ cells from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.

Application References
  1. Arase H, et al. 2001. J. Immunol. 167:1141. (FC)
  2. Sepulveda H, et al. 1999. J. Immunol. 163:1133.
  3. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  4. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  5. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
  7. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  8. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
  9. Busche A, et al. 2011. J. Immunol. 186:2918. PubMed
  10. Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed
  11. Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed
  12. Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed
  13. Honjo K, et al. 2012. PNAS. PubMed.
  14. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed
Product Citations
  1. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  2. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  3. Rosenheinrich M, et al. 2015. PLoS One. 10: 0136290. PubMed
  4. Elsner R, et al. 2015. PLoS Pathog. 10: 1004976. PubMed
  5. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  6. Guo F, et al. 2022. Front Immunol. 13:833100. PubMed
  7. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  8. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  9. Li W, et al. 2012. J Virol. 86:12304. PubMed
  10. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  11. Cravens P, et al. 2016. PLoS One. 11:e0165248. PubMed
  12. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
  13. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  14. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  15. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  16. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  17. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  18. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  19. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  20. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  21. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  22. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  23. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  24. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  25. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  26. Iki M, et al. 2016. Blood. 128(25):2909-2918. PubMed
  27. Tsutsui H, et al. 2017. J Biol Chem. 292(3):1061-1067. PubMed
  28. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  29. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  30. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  31. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  32. Pisano F, et al. 2014. PLoS One. 9:103541. PubMed
  33. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  34. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  35. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  36. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
RRID
AB_313410 (BioLegend Cat. No. 108903)
AB_313411 (BioLegend Cat. No. 108904)

Antigen Details

Structure
Integrin α chain, 150 kD
Distribution

NK cells, subset of T cells

Function
Adhesion
Ligand/Receptor
Collagen, laminin
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Arase H, et al. 2001. J. Immunol. 167:1141.
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
3. Sasaki K, et al. 2003. Int. Immunol. 15:701.
4. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account