Biotin anti-BrdU Antibody

Pricing & Availability
MoBU-1 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Brdu-incorporated Hut-78 cells stained with biotinylated MoBU-1, followed by Sav-PE
  • MoBU-1_Biotin_020808
    Brdu-incorporated Hut-78 cells stained with biotinylated MoBU-1, followed by Sav-PE
Cat # Size Price Quantity Check Availability Save
317904 100 µg 155€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. The MoBU-1 antibody is useful for the detection of incorporated BrdU by fluorescent cell staining (ICFC), flow cytometry, and immunohistochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Jung H, et al. 2012. J Exp Med. 209:2409. PubMed
Product Citations
  1. Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed
  2. Jung H, et al. 2012. J Exp Med. 209:2409. PubMed
AB_604041 (BioLegend Cat. No. 317904)

Antigen Details

Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.

Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.

Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area
Cell Biology, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.

Gene ID
View information about BrdU on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All BrdU Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-BrdU MoBU-1 FC, ICFC, IHC-F
Biotin anti-BrdU MoBU-1 ICFC
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-BrdU

  • Biotin anti-BrdU


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account