APC/Cyanine7 anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_APCCyanine7_HLA-DR_Antibody_010719_updated.png
Human peripheral blood lymphocytes were stained with CD19 FITC and HLA-DR (clone L243) APC/Cyanine7 (left), or mouse IgG2a, ? APC/Cyanine7 isotype control (right)
  • L243_APCCyanine7_HLA-DR_Antibody_010719_updated.png
    Human peripheral blood lymphocytes were stained with CD19 FITC and HLA-DR (clone L243) APC/Cyanine7 (left), or mouse IgG2a, ? APC/Cyanine7 isotype control (right)
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
307617 25 tests 104€
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307618 100 tests 212€
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Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Chimpanzee, Cynomolgus, Dog (Canine), Marmoset, Rhesus, Squirrel Monkey, Tamarin
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The HLA-DR antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, and immunohistochemical staining of acetone-fixed frozen sections4,5. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
Product Citations
  1. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  2. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  3. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  4. Vaccari M, et al. 2018. Nat Med. 24:847. PubMed
  5. Kristoff J, et al. 2019. EBioMedicine. 43:295. PubMed
  6. Vaccari M, et al. 2019. Front Immunol. 10:1072. PubMed
  7. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  8. Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
  9. Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
  10. Wosen JE, et al. 2019. Front Immunol. 1.784722222. PubMed
  11. Hakimi AA, et al. 2019. Cancer Discov. 9:510. PubMed
  12. Gorini G, et al. 2020. PLoS Pathog. 16:e1008377. PubMed
  13. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  14. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  15. D'Antoni ML, et al. 2019. Front Immunol. 2.353472222. PubMed
  16. Rodrigues E, et al. 2020. Nat Commun. 3.99375. PubMed
  17. Demirel &, et al. 2007. J Biol Chem. 282:37836. PubMed
  18. Everts B, et al. 2010. PLoS One. 4:e667. PubMed
  19. Donini M, et al. 2011. Prostate. 72:566. PubMed
  20. Reismann M, et al. 2014. J Surg Res. 187:197. PubMed
  21. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  22. Teirlinck A, et al. 2015. Infect Immun . 83: 3732-3739. PubMed
  23. Kurose K, et al. 2015. Clin Cancer Res. 21: 4327 - 4336. PubMed
  24. Vogel S, et al. 2015. Sci Rep. 5: 18308. PubMed
  25. Finotti G, et al. 2016. J Leukoc Biol. 99: 107 - 119. PubMed
  26. Nicholas K, et al. 2015. Cytometry A. 10.1002/cyto.a.22799. PubMed
  27. Lundberg K, et al. 2016. PLoS One. 11: 0148838. PubMed
  28. Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed
  29. Castro-Amarante M, et al. 2016. J Virol. 90: 2195 - 2207. PubMed
  30. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
  31. Pribyl M, et al. 2020. Mol Oncol. 2.252083333. PubMed
  32. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  33. Swanson E, et al. 2021. eLife. 10:00. PubMed
  34. Takiguchi H, et al. 2021. Scientific Reports. 11(1):8282. PubMed
  35. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
RRID
AB_493587 (BioLegend Cat. No. 307617)
AB_493586 (BioLegend Cat. No. 307618)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/07/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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