APC anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_APC_1_110210
C57BL/6 mouse splenocytes were stained with CD49b (DX5) FITC and NK1.1 (clone PK136) APC (top) or mouse IgG2a APC isotype control (bottom).
  • PK136_APC_1_110210
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) FITC and NK1.1 (clone PK136) APC (top) or mouse IgG2a APC isotype control (bottom).
  • PK136_APC_2_110210
See APC spectral data
Cat # Size Price Quantity Avail. Save
108709 25 µg 64,00€
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108710 100 µg 212,00€
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The NK-1.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, and immunofluorescence microscopy11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
Product Citations
  1. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  2. Neumann AM et al. 2017. PloS one. 12(11):e0187330 . PubMed
  3. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  4. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  5. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  6. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  7. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  8. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  9. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  10. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  11. Shen T, et al. 2019. Cell Death Discov. 5:123. PubMed
  12. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  13. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  14. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  15. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  16. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  17. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  18. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  19. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  20. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  21. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  22. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  23. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  24. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  25. Liu W, et al. 2020. Mol Ther Oncolytics. 17:350. PubMed
  26. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  27. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  28. Huang W, et al. 2014. J Leukoc Biol. 96:55. PubMed
  29. Textor A, et al. 2014. Cancer Res. 74:6769. PubMed
  30. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  31. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  32. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  33. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  34. Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed
  35. Hirata Y, et al. 2015. PLoS One. 10: 0141785. PubMed
  36. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  37. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  38. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  39. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  40. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  41. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  42. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  43. Janakiram N, et al. 2016. Sci Rep. 6:37046. PubMed
  44. Zhang H, et al. 2017. Immunol Cell Biol. 10.1038/icb.2017.11. PubMed
  45. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  46. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  47. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  48. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  49. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
RRID
AB_313396 (BioLegend Cat. No. 108709)
AB_313397 (BioLegend Cat. No. 108710)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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