APC anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
5 reviews
Product Citations
publications
1A8_APC_091809
C57BL/6 bone marrow cells stained with 1A8 APC
  • 1A8_APC_091809
    C57BL/6 bone marrow cells stained with 1A8 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
127613 25 µg 64€
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127614 100 µg 228€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Gottfried-Blackmore A, et al. 2009. Proc Natl Acad Sci U S A. 106:20918. PubMed
  2. Oertli M, et al. 2011. J Immunol. 187:3578. PubMed
  3. Kamei A, et al. 2012. J Infect Dis. 207:39. PubMed
  4. Devi S, et al. 2013. J Exp Med. 210:2321. PubMed
  5. Sharma A, et al. 2014. J Infect Dis. . PubMed
  6. Patnode M, et al. 2014. J Exp Med. 211:1281. PubMed
  7. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
  8. Christian D, et al. 2014. Infect Immun . 82:4056. PubMed
  9. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  10. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  11. Price P, et al. 2015. J Immunol. 194:1164. PubMed
  12. Steichen A, et al. 2015. PLoS One. 10:123573. PubMed
  13. Gardner P, et al. 2015. Am J Pathol. 185:1324. PubMed
  14. Liao H, et al. 2015. Rheumatology. 54: 1317 - 1326. PubMed
  15. Cardoso F, et al. 2015. PLoS One. 10: 0131904. PubMed
  16. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
  17. Weiss M, et al. 2015. Proc Natl Acad Sci U S A. 112: 11001-11006. PubMed
  18. Patankar Y, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L902 - L913. PubMed
  19. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  20. Ma Y, et al. 2016. Cardiovasc Res. 110: 51 - 61. PubMed
  21. Ioannidis L, et al. 2016. J Immunol. 196: 1227 - 1238. PubMed
  22. Karmakar M, et al. 2016. Nat Commun. 7:10555. PubMed
  23. Stein K, et al. 2016. J Leukoc Biol. 99: 231 - 239. PubMed
  24. Chu C, et al. 2016. Dis Model Mech. 9: 473-481. PubMed
  25. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
  26. Heuslein J, et al. 2016. Sci Rep. 6: 27029. PubMed
  27. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  28. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  29. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  30. Chua Y, et al. 2016. J Immunol. 197: 3771 - 3781. PubMed
  31. Peñaloza HF, et al. 2018. Biochem Biophys Rep. 13:12. PubMed
  32. Ohno-Urabe S, et al. 2018. J Am Heart Assoc. 7:e007389. PubMed
  33. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  34. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  35. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  36. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  37. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  38. Parlet CP, et al. 2019. Cell Rep. 27:187. PubMed
  39. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  40. Patras KA, et al. 2019. J Innate Immun. :1. PubMed
  41. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  42. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  43. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  44. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  45. Li YT, et al. 2019. Elife. 8:e47642. PubMed
  46. Resende M, et al. 2019. J Immunol. 203:2451. PubMed
  47. Vasamsetti SB et al. 2018. Immunity. 49(1):93-106 . PubMed
  48. Wang SS et al. 2018. Cell chemical biology. 25(12):1519-1532 . PubMed
  49. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  50. Wingrove E et al. 2019. Cell reports. 27(4):1277-1292 . PubMed
  51. Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed
  52. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  53. Janela B et al. 2019. Immunity. 50(4):1069-1083 . PubMed
  54. Zhang J et al. 2019. Cell Rep. 27(9):2679-2689 . PubMed
  55. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  56. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  57. Kobayashi Y, et al. 2018. PLoS One. 13:e0195008. PubMed
  58. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  59. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  60. Li H, et al. 2019. Front Immunol. 2.205555556. PubMed
RRID
AB_1877163 (BioLegend Cat. No. 127613)
AB_2227348 (BioLegend Cat. No. 127614)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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