- YTS156.7.7 (See other available formats)
- Other Names
- Lyt-3, Ly-3
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.Product Details
- Antibody Type
- Host Species
- Mouse thymocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
- Application References
- McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
- Product Citations
AB_2563948 (BioLegend Cat. No. 126617)
AB_2563949 (BioLegend Cat. No. 126618)
- Ig superfamily, CD8β chain, 34 kD
Majority of thymocytes, cytotoxic T cell subsets
- Co-receptor of TCR
- MHC class I
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
- Gene ID
- 12526 View all products for this Gene ID
- View information about CD8b on UniProt.org
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