- 10D8 (See other available formats)
- Other Names
- α-tubulin, Tubulin, alpha-ubiquitous chain
- Mouse IgM, κ
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- Product Citations
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Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.Product Details
- Human, Mouse, Rat, All Species
- Antibody Type
- Host Species
- Recombinant full length human α-tubulin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IF - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.25-5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Red Laser (633 nm)
- Application References
- Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
AB_2563178 (BioLegend Cat. No. 627908)
- Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
- Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
- RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
- Phosphorylation, acetylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Antigen References
1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.
- Gene ID
- 84790 View all products for this Gene ID
- View information about Tubulin-alpha on UniProt.org
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