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Alexa Fluor® 647 anti-rat IgG2a Antibody

Pricing & Availability
Clone
MRG2a-83 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Mouse IgG
Ave. Rating
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Product Citations
publications
MRG2a-85_A647_IgG2b_Antibody_FC_071218
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 647 .
  • MRG2a-85_A647_IgG2b_Antibody_FC_071218
    C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 647 .
  • MRG2b-85_A647_IgG2b_Antibody_IHC_100217
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD8a (clone 53-6.7) purified and 10 µg/ml of CD20 (clone SA275A11) Brilliant Violet 421™ (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG2a (Clone MRG2a-83) Alexa Fluor® 647 (red) for 2 hours at room temperature. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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407511 25 µg 72€
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407512 100 µg 156€
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Description

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
Product Citations
  1. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  2. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
  3. Park JY, et al. 2019. Theranostics. 9:3398. PubMed
RRID
AB_2716139 (BioLegend Cat. No. 407511)
AB_2716140 (BioLegend Cat. No. 407512)

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Go To Top Version: 0    Revision Date: 10/03/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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