- 14-4-4S (See other available formats)
- Other Names
- I-Ek, MHC class II
- Mouse (C3H.SW) IgG2a, κ
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The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.Product Details
- Mouse, Cross-reacts with H-2d,p,r haplotypes. Mice of the H-2b,f,q,s haplotypes do not express I-E antigens. Cross-reacts with rat MHC class II antigen RT1D
- Antibody Type
- Host Species
- C3H mouse skin graft and splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC, IF - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.
- Application References
- Ozato K, et al. 1980. J. Immunol. 124:533.
- Nei U, et al. 1994. Int. Immunol. 6:61. (IP)
- Harton JA, et al. 1991. Immunogenetics 34:358.
- Roy M, et al. 1995. Eur. J. Immunol. 25:596.
- Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC)
- Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC)
- Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
AB_493214 (BioLegend Cat. No. 110211)
- MHC class II
- B cell and activated T cells, APCs of H-2b mice
- Antigen presentation
- CD3/TCR, CD4
- Cell Type
- B cells, T cells, Antigen-presenting cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
- Gene ID
- 14969 View all products for this Gene ID
- View information about I-Ek on UniProt.org
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