Alexa Fluor® 647 anti-mouse I-Ek/rat RT1D Antibody

Pricing & Availability
Clone
14-4-4S (See other available formats)
Other Names
I-Ek, MHC class II
Isotype
Mouse (C3H.SW) IgG2a, κ
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Product Citations
publications
14-4-4s_Alx647_020608
C3H/He mouse splenocytes stained with 14-4-4S Alexa Fluor® 647
  • 14-4-4s_Alx647_020608
    C3H/He mouse splenocytes stained with 14-4-4S Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
110211 25 µg 68€
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Description

The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Cross-reacts with H-2d,p,r haplotypes. Mice of the H-2b,f,q,s haplotypes do not express I-E antigens. Cross-reacts with rat MHC class II antigen RT1D
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC, IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.

Application References
  1. Ozato K, et al. 1980. J. Immunol. 124:533.
  2. Nei U, et al. 1994. Int. Immunol. 6:61. (IP)
  3. Harton JA, et al. 1991. Immunogenetics 34:358.
  4. Roy M, et al. 1995. Eur. J. Immunol. 25:596.
  5. Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC)
  6. Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
RRID
AB_493214 (BioLegend Cat. No. 110211)

Antigen Details

Structure
MHC class II
Distribution
B cell and activated T cells, APCs of H-2b mice
Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
B cells, T cells, Antigen-presenting cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14969 View all products for this Gene ID
UniProt
View information about I-Ek on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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