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Alexa Fluor® 647 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
1_BM8_A647_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 647.
  • 1_BM8_A647_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 647.
  • 2_BM8_A647_F4slash80_Antibody_2_IHCF_120320
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-mouse F4/80 (clone BM8) Alexa Fluor® 647 (red), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (blue), and anti-mouse/rat/human CD27 (clone LG.3A10) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured with a 10X objective.
  • 3_BM8_A647_F4-80_Antibody_2_092121.png
    Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD146 Antibody (clone ME-9F1) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-mouse F4/80 Antibody (clone BM8) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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123121 25 µg 118€
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123122 100 µg 259€
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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References
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  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
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  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  15. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  16. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  17. Chan IS, et al. 2020. J Cell Biol. :219. PubMed
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  19. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  20. Hu K, et al. 2015. PLoS One. 10: 0137123. PubMed
  21. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  22. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  23. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  24. Chen S, et al. 2021. Immunology. 163:201. PubMed
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  27. Dean JM, et al. 2020. Cell Rep. 33:108228. PubMed
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  29. Yang Y, et al. 2016. Nat Commun. 7:11385. PubMed
  30. Galvani E, et al. 2020. Nat Commun. 11:853. PubMed
  31. Petrus P, et al. 2020. Cell Metabolism. 31(2):375-390.e11.. PubMed
  32. Dobrian A, et al. 2011. Am J Physiol Endocrinol Metab. 300:E410. PubMed
  33. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
  34. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  35. Jaworska K, et al. 2015. J Immunol. 194:325. PubMed
  36. Shankar J 2016. Innate Immunity. 22: 31 - 39. PubMed
  37. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  38. Belliere J, et al. 2015. J Am Soc Nephrol. 26:1363. PubMed
  39. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
  40. Ruiz–Manzano RA, et al. 2020. Frontiers in Oncology. 0.927777778. PubMed
  41. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  42. Daemen S, et al. 2021. STAR Protoc. 2:100511. PubMed
  43. Rani GF, et al. 2021. Wellcome Open Res. 6:83. PubMed
  44. Tavares J, et al. 2013. J Exp Med. 210:905. PubMed
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  46. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  47. Qiao S, et al. 2019. J Neuroinflammation. 16:4. PubMed
  48. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  49. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  50. He L, et al. 2021. Cell Rep. 37:109955. PubMed
  51. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  52. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  53. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  54. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  55. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  56. Hosaka K, et al. 2020. Nat Commun. 3.030555556. PubMed
  57. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-00654-2. PubMed
  58. Garg BK, et al. 2019. PLoS One. 14:e0214942. PubMed
  59. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  60. Wang S, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.104. PubMed
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  62. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  63. Wang J, et al. 2015. Cancer Res. 75:306. PubMed
  64. Bai WJ, et al. 2018. Front Immunol. 8:1919. PubMed
  65. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  66. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
  67. Zeid AM, et al. 2022. FASEB J. 36:e22286. PubMed
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  69. Woods PS, et al. 2020. Am J Respir Cell Mol Biol. 62:243. PubMed
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  72. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
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RRID
AB_893492 (BioLegend Cat. No. 123121)
AB_893480 (BioLegend Cat. No. 123122)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 09/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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