Alexa Fluor® 647 anti-human CD337 (NKp30) Antibody

Pricing & Availability
P30-15 (See other available formats)
Regulatory Status
Other Names
NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with P30-15 Alexa Fluor® 647
  • P30-15_Alx647_072407
    Human peripheral blood lymphocytes stained with P30-15 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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325212 100 tests 235€
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The p30-15 monoclonal antibody recognizes CD337 also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3. NKp30 is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp30 has an apparent molecular weight of 30 kD and six isoforms are produced by alternative splicing. NKp30 is expressed on resting and activated NK cells. NKp30 enhances NK cell cytolysis of tumor cellts that are deficient in MHC class I molecules. NKp30 has been shown to associate with CD59 and TCRζ. The p30-15 antibody against human NKp30 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
Recombinant human NKp30
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution1,3,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).

Application References
  1. Stark S, et al. 2005. J. Immunol. Methods 296:149. (Block)
  2. Mansater I, et al. 2008. J. Immunol. 181:1869. PubMed
  3. Markel G, et al. 2009. PLOS One 4:e5597. (Block)
  4. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  5. Byrd A, et al. 2007. PLoS One. 2:e1339. (Block)
Product Citations
  1. Cordeau M, et al. 2016. PLoS One. 11:e0164401. PubMed
  2. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  3. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
  4. Karampatzakis A, et al. 2021. Front Immunol. 12:641521. PubMed
  5. El Costa H, et al. 2021. PLoS Pathog. 17:e1009367. PubMed
  6. Damele L, et al. 2021. Cancers (Basel). 13:. PubMed
  7. Hood SP, et al. 2020. Elife. 9:00. PubMed
  8. Hearps AC, et al. 2017. Front Immunol. 0.840972222. PubMed
  9. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
AB_2149448 (BioLegend Cat. No. 325212)

Antigen Details

Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.

IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro

NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
CD59, TCRζ
Cell Type
NK cells, T cells
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Pende D, et al. 1999. J. Exp. Med. 190:1505.

Gene ID
259197 View all products for this Gene ID
View information about CD337 on

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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