Alexa Fluor® 647 anti-human CD209 (DC-SIGN) Antibody

Pricing & Availability
Clone
9E9A8 (See other available formats)
Regulatory Status
RUO
Other Names
Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
9E9A8_A647_090808.jpg
Human monocytes-derived dendritic cells were stained with CD209 (clone 9E9A8) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 9E9A8_A647_090808.jpg
    Human monocytes-derived dendritic cells were stained with CD209 (clone 9E9A8) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 9E9A8_AF647_CD209_Antibody_IF_011414
    Human peripheral blood mononuclear cell (PBMC) derived dendritic cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of CD209 (DC-SIGN) (clone 9E9A8) Alexa Fluor® 647 for 30 minutes at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured by 40X objective.
See Alexa Fluor® 647 spectral data See high resolution ICC data...
Cat # Size Price Quantity Check Availability Save
330111 25 tests 84,00€
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330112 100 tests 180,00€
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Description

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human DC-SIGN
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1.

Application References
  1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F)
Product Citations
  1. Surdziel E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183679. PubMed
  2. Pagan JD, et al. 2018. Cell. 172:564. PubMed
  3. Olagnier D, et al. 2014. PLoS Pathog. 10:1004566. PubMed
  4. Prasad A, et al. 2017. Sci Rep. 7:40648. PubMed
RRID
AB_1186094 (BioLegend Cat. No. 330111)
AB_1186092 (BioLegend Cat. No. 330112)

Antigen Details

Distribution

Dendritic cells

Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.

Gene ID
30835 View all products for this Gene ID
UniProt
View information about CD209 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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