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Alexa Fluor® 594 anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
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Product Citations
publications
A-8-1-1_AF594_Podoplanin_Antibody_ICC_042419
TE-71 mouse thymic epithelial stromal cell line cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml podoplanin (clone 8.1.1) Alexa Fluor® 594 (red) in blocking buffer at room temperature for 3 hours. Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • A-8-1-1_AF594_Podoplanin_Antibody_ICC_042419
    TE-71 mouse thymic epithelial stromal cell line cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/ml podoplanin (clone 8.1.1) Alexa Fluor® 594 (red) in blocking buffer at room temperature for 3 hours. Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • B-8-1-1_AF594_Podoplanin_Antibody_IHC-F_042419
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml CD100 (clone BMA-12) purified and podoplanin (clone 8.1.1) Alexa Fluor® 594 (red) overnight at 4°C, followed by 2.5 µg/ml of goat anti rat IgG (clone Poly4054) Alexa Fluor® 647 (green) for 2 hours at room temperature. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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127414 100 µg 278€
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Application References
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  2. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  3. Bunse M, et al. 2021. Nat Commun. 0.666666667. PubMed
RRID
AB_2563351 (BioLegend Cat. No. 127414)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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