- Clone
- G10F5 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI MA81
- Other Names
- CD67, CGM6, NCA-95, CEACAM8
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered Saline 1X (pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 (Cat. No. 925501) twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 647 anti-CD45RA (clone HI100) antibody (green), Alexa Fluor® 594 anti-CD66b (clone G10F5) antibody (red) and Brilliant Violet 421™ anti-CD45RO antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 305123 | 25 µg | 108,00€ | ||||
| 305124 | 100 µg | 260,00€ | ||||
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
Product DetailsProduct Details
- Reactivity
- Human, Cross-Reactivity: Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen and formalin-fixed paraffin-embedded tissue sections.
- Application References
-
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
- Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
- RRID
-
AB_2728242 (BioLegend Cat. No. 305123)
AB_2728243 (BioLegend Cat. No. 305124)
Antigen Details
- Structure
- Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
- Distribution
- Granulocytes
- Function
- Cell adhesion, neutrophil activation
- Ligand/Receptor
- CD66c
- Cell Type
- Granulocytes, Neutrophils
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551. - Gene ID
- 1088 View all products for this Gene ID
- UniProt
- View information about CD66b on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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