Product: Pacific Blue™ anti-mouse Lineage Cocktail |
Catalog No.: 133310 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining Mouse Bone Marrow with anti-lineage Cocktail in Pacific Blue™ | |||
Overall: | Product Quality: | Ease of Use: | |
Pacific Blue™ is a great fluorochrome to use for marking lineage positive cells in mouse bone marrow. |
Experimental Design |
Application: | Flow cytometry - Surface staining |
Cells used: | Mouse bone marrow |
Brief Protocol: | Bone marrow was flushed from femurs and tibiae with PBS/2%FBS through a 27 gauge needle and a 40uM strainer. Cells were washed, counted, and stained for 20 minutes at room temperature with 20uL per test anti-lineage Pacific Blue. Cells were washed twice and analyzed on a BD LSR II. |
Results Summary: | Lineage staining was excellent. |
Additional Notes: |
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