Product: PE anti-human Ganglioside GD2

Catalog No.: 357303

Brian Bierie, Postdoc WIBR

GD2 Detection
Overall:	Product Quality:	Ease of Use:
GD2 was detected at the surface of mesenchymal carcinoma cell lines.

Experimental Design

Application:	FACS
Cells used:	SUM159
Brief Protocol:	Cells were treated with trypsin and quenched using DMEM + 10% FBS. Pellets were re-suspended at 1x10^6 cells per 100 ul of ice cold PBS + 2% IFS. Antibody was used at 1:100 and incubated on ice for 30 minutes. Cells were then washed with a 10:1 volume of ice cold PBS + 2% IFS. Pellets were re-suspended in ice cold PBS + 2% IFS to a volume of 500 ul.
Results Summary:	Approximately 17% of the cells were GD2 high
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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