Product: Zombie UV™ Fixable Viability Kit |
Catalog No.: 423108 |
Sheila Rao, Post-doc Salk Institute |
Zombie UV™ | |||
Overall: | Product Quality: | Ease of Use: | |
Zombie UV nicely separates live and dead cells in mouse tissue. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mesenteric lymph nodes |
Brief Protocol: | 1. Wash cells in PBS. 2. Dilute Zombie-UV 1:800 in PBS. 3. R/S cells in 100ul Zombie-UV. 4. Incubate 20 minutes on ice, covered. 5. Wash with FACS (PBS, 2% FBS) and proceed to surface stain. |
Results Summary: | Dead cells can easily be distinguished using Zombie UV and FSC. |
Additional Notes: |
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