Product: PE anti-human Ganglioside GD2

Catalog No.: 357303

Sarah Pierce, Undergraduate WIBR

GD2 (PE anti-human Ganglioside)
Overall:	Product Quality:	Ease of Use:
GD2 as a surface marker on mesenchymal cell lines.

Experimental Design

Application:	Flow cytometry
Cells used:	SUM159
Brief Protocol:	Cells were trypsinized, neutralized with DMEM 10%, and re-suspended in ice-cold PBS with 2% IFS at 1x10^6 cells per 100 uL. The cells were incubated on ice with 1:100 antibody for 30 min, washed with 9 times the original volume of ice-cold PBS- with 2% IFS, and re-suspended in 500 uL ice-cold PBS- with 2% IFS.
Results Summary:	10.3% of the cells were GD2 high.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

Trypsinization can affect epitopes as it is enzyme-based. This can make it difficult for antibodies to recognize their targets.

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