Product: Alexa Fluor® 647 anti-human CD31

Catalog No.: 303111

Chris Corkum, Research Assistant Memorial University

Excellent for Staining Peripheral Blood Mononuclear Cells by Flow Cytometry (Alexa Fluor® 647 anti-human CD31)
Overall:	Product Quality:	Ease of Use:
This antibody resulted in bright staining and easy identification of CD31-positive cells from human PBMC.

Experimental Design

Application:	Flow Cytometry
Cells used:	human PBMC isolated from whole blood by Ficoll
Brief Protocol:	Approximately 1x10^6 cells were incubated with Alexa Fluor 647-conjugated anti-human CD31 antibody at a dilution of 1:20 (0.5ug) for 1 hour on ice in the dark in a total volume of ~100ul FACS buffer (PBS+2% FCS, 1mM EDTA, 0.1% sodium azide). Following incubation, cells were washed twice with FACS buffer and immediately analysed.
Results Summary:	Cells stained with anti-CD31 are shown in the bottom panel, where CD31-positive cells are easily distinguished from non-expressing cells. Cells incubated with an Alexa Fluor 647-conjugated matched isotype control are displayed in the top panel.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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