Antibodies can be used for a variety of functional assays, including blocking, activation, depletion, or neutralization, for both in vitro and in vivo purposes. It is critical that these reagents are free of preservatives, such as sodium azide, and have very low levels of endotoxin. BioLegend offers biofunctional antibodies in three product formats: LEAF™ (Low Endotoxin, Azide-Free), Ultra-LEAF™, and GoInvivo™. Compare the formats below.

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  GoInVivo™ Ultra-LEAF™ LEAF™
Endotoxin Level < 1 EU/mg of protein < 10 EU/mg of protein < 100 EU/mg of protein
Sizing Options 5 mg - 1 g 100 µg - 25 mg 50 µg- 1 mg
QC Testing Flow Cytometry Flow Cytometry Flow Cytometry
Pricing Best value per mg Better value per mg Good value
Pathogen Testing Yes No No
Functional Validation Yes No No
Number of Products 20+ 100+ 320+
Notes Focused on immune checkpoint receptor antibodies Excellent for in vivo administration  

 

Biofunctional Resources

Featured Products


 

GoInVivo™ Purified anti-mouse CD274 (B7-H1, PD-L1)

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CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

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Treg-Protector™ (anti-ARTC2 Nanobody)

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ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.

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LEAF™ Purified anti-mouse DR3 (TNFRSF25)

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DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.

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Ultra-LEAF™ Purified anti-mouse CX3CR1

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CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

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Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2)

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CD182, also known as CXCR2, is a G-protein coupled receptor with seven transmembrane regions that is involved in chemotaxis, neutrophil activation, and angiogenesis. CXCR2 is expressed by neutrophils, basophils, a subset of T cells, monocytes, macrophages, natural killer (NK) and natural killer T cells (NKT). Its ligands are CXCL1, CXCL2, CXCL3, and CXCL5.

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