Determining cross-reactivity of the clones included in the Human LEGENDScreen™ kit with each of the non-human primate species is only one factor important when preparing for an assay. Identifying any differences or changes in the patterns of expression for each marker on different hematologic cell subsets is equally important for planning an assay or interpreting the results. Since all of the antibodies offered in LEGENDScreen™ are conjugated to phycoerythrin (PE), a panel of backbone markers listed in Table 1. can be employed on complementary fluorophores to PE to phenotype the different subsets. These are clones with known cross-reactivity and expression patterns to best phenotype granulocytes, NK cells, B-cells , T-cells and monocytes in all the NHP species tested. Analysis was done using Cytobank software.