While there are tradeoffs to all three of the methods mentioned above, each provides a unique way to standardize your flow cytometer. These baseline settings will help monitor for any changes or issues that might occur due to problems associated with the laser, electronic noise, filters, and PMTs. Any swings in these voltages should be investigated further. Lastly, it's important to remember that these baseline settings are not meant to be experiment-specific. However, following these protocols will set you on the right track to producing reliable, consistent data with your flow cytometer. Stay tuned for a follow-up blog that delves into voltage and PMT settings for experiments.
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