FITC anti-human CD3 Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4,6,7 and formalin-fixed paraffin-embedded sections¹¹, immunoprecipitation¹, activation of T cells^2,3,5, Western blotting⁹, and spatial biology (IBEX)^16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)

1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
2. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
3. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
4. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
5. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
6. Carre C, et al. 2021. iScience. 24:102970. PubMed
7. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
8. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
9. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
10. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
11. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
12. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
13. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
14. Li C, et al. 2022. J Exp Med. 219:. PubMed
15. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
16. Chen L, et al. 2018. Immunology. 155:123. PubMed
17. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
18. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
19. Laustsen A, et al. 2021. Elife. 10:. PubMed
20. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
21. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
22. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
23. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
24. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
25. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
26. Ruetsche D, et al. 2022. Cells. 11:. PubMed
27. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
28. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
29. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
30. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
31. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
32. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
33. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
34. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
35. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
36. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
37. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
38. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
39. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
40. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
41. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
42. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
43. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
44. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
45. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
46. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
47. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
48. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
49. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
50. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
51. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
52. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
53. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
54. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
55. Coufal S, et al. 2019. Cells. 8. PubMed
56. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
57. Platten M, et al. 2021. Nature. 592:463. PubMed
58. Jin S, et al. 2020. Cell Res. 30:950. PubMed
59. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
60. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
61. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
62. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
63. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
64. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
65. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
66. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
67. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
68. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
69. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
70. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
71. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
72. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
73. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
74. Abe Y, et al. 2022. Nat Cell Biol. 24:565. PubMed
75. Cipriano M, et al. 2022. Commun Biol. 5:52. PubMed
76. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
77. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
78. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
79. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
80. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
81. Wang Z, et al. 2021. Nat Commun. 12:1724. PubMed
82. Capone S, et al. 2020. NPJ Vaccines. 5:94. PubMed
83. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
84. van Erp EA, et al. 2020. Clin Transl Immunology. 9:e1112. PubMed
85. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
86. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
87. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
88. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
89. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
90. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
91. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
92. Albrecht M, et al. 2022. Clin Transl Immunology. 11:e1398. PubMed
93. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
94. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
95. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
96. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
97. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
98. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
99. Pappa V, et al. 2021. J Neuroimmunol. 353:577524. PubMed
100. Vanoni G, et al. 2021. eLife. 10:00. PubMed
101. Goc J, et al. 2021. Cell. 184:5015. PubMed
102. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
103. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
104. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
105. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed

RRID

AB_314059 (BioLegend Cat. No. 300405)
AB_314060 (BioLegend Cat. No. 300406)
AB_2564148 (BioLegend Cat. No. 300452)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure

Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD

Distribution

Mature T and NK T cells, thymocyte differentiation

Function

Antigen recognition, signal transduction, T cell activation

Ligand/Receptor

Peptide antigen bound to MHC

Cell Type

NKT cells, T cells, Thymocytes, Tregs

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, TCRs

Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID

916 View all products for this Gene ID

UniProt

View information about CD3 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis