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Purified anti-β-actin Antibody

Pricing & Availability
Clone
Poly6221 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6221_PURE_betaactin_Antibody_1_WB_090616
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_1_WB_090616
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_2_IF_062016
    Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
  • poly6221_PURE_betaactin_Antibody_3_IHCP_102720
    Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6221 and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
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622101 50 µl (5 Western blots) 95€
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622102 200 µl (20 Western blots) 235€
 
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH, NH2 terminus
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1:100~1:400. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.PubMed
  4. KishidaT,et al.2007.J. Immunol. 179:8554.PubMed
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  15. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
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Product Citations
  1. Checa M, et al. 2016. PLoS One. 11: 0150383. PubMed
  2. Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed
  3. Al–Naggar IM, et al. 2019. J Gerontol A Biol Sci Med Sci. 74:325. PubMed
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  6. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  7. Wang D, et al. 2018. Immunity. 48:659. PubMed
  8. Agabiti S, Li J, Wiemer A 2017. Cell Death Dis. 10.1038/cddis.2017.101. PubMed
  9. Wakatsuki S, et al. 2021. J Cell Biol. 220:. PubMed
  10. Piao X, et al. 2012. Sci Signal. 5:93. PubMed
  11. Kartika ID, et al. 2021. Cancer Med. 10:8988. PubMed
  12. Harvey B, et al. 2014. Cytokine. 65:236. PubMed
  13. Wakatsuki S, et al. 2015. J Cell Biol. 211: 881 - 896. PubMed
  14. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  15. Pfeil EM, et al. 2020. Molecular Cell. 80(6):940-954.e6. PubMed
  16. Hua X, et al. 2015. PLoS One. 10:128039. PubMed
  17. Che L, et al. 2019. Exp Ther Med. 18:1745. PubMed
  18. Ouimet M, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1144. PubMed
  19. Rahman M, et al. 2010. J Immunol. 184:7247. PubMed
  20. Takeda K, et al. 2017. Nat Commun. 8:14607. PubMed
  21. Toltl L, et al. 2008. J Immunol. 181:2165. PubMed
  22. Tao HC, et al. 2020. Front Immunol. 11:148. PubMed
  23. Man F, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L803. PubMed
  24. Taha A, et al. 2020. J Cell Physiol. 7094:235. PubMed
  25. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  26. Berdasco C, et al. 2020. Front Cell Infect Microbiol. 0.681944444. PubMed
  27. Feddersen CR, et al. 2019. Cancer Res. 79:5074. PubMed
  28. Joyce C, et al. 2006. J Biol Chem. 281:33053. PubMed
  29. Zhu S et al. 2017. Cancer cell. 32(3):310-323 . PubMed
  30. Naneix F et al. 2017. eNeuro. 4(3) pii: ENEURO. PubMed
  31. Yanagiya T, et al. 2007. Obesity . 1.022222222. PubMed
  32. Kishida T, et al. 2007. J Immunol. 179:8554. PubMed
  33. Zheng X, et al. 2018. Int J Mol Med. 128:1300. PubMed
  34. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  35. Caglar Beker M, et al. 2020. Turk J Med Sci. Online ahead of print. PubMed
  36. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:F70. PubMed
  37. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  38. Fliegauf M, et al. 2021. Front Immunol. 12:621503. PubMed
  39. Ng C, et al. 2013. J Virol. 87:9511. PubMed
  40. Grundmann M, et al. 2018. Nat Commun. 9:341. PubMed
  41. Sawada T, et al. 2008. J Biol Chem. 283:26820. PubMed
  42. Pototskiy E, et al. 2021. Sci Rep. 11:17262. PubMed
  43. Tsurusaki S, et al. 2019. Cell Death Dis. 10:449. PubMed
  44. Panwar P, et al. 2018. Br J Pharmacol. 175:902. PubMed
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RRID
AB_315945 (BioLegend Cat. No. 622101)
AB_315946 (BioLegend Cat. No. 622102)

Antigen Details

Structure
Member of the actin family; 42 KD
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Interaction
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Regulation
Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 10.27.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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