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APC anti-mouse IL-3 Antibody

Pricing & Availability
Clone
MP2-8F8 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MP2-8F8_APC_IL-3_Antibody_051019.png
PMA+Ionomycin stimulated (six hours, in presence of brefeldin A) Th2-polarized C57BL/6 T cells were stained with surface CD4 PE/Cyanine7. Cells were fixed and permeablized and then stained intracelluarly with anti-mouse IL-3 (clone MP2-8F8) APC (left) or Rat IgG1, κ APC isotype control (right).
  • MP2-8F8_APC_IL-3_Antibody_051019.png
    PMA+Ionomycin stimulated (six hours, in presence of brefeldin A) Th2-polarized C57BL/6 T cells were stained with surface CD4 PE/Cyanine7. Cells were fixed and permeablized and then stained intracelluarly with anti-mouse IL-3 (clone MP2-8F8) APC (left) or Rat IgG1, κ APC isotype control (right).
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503907 25 µg 86€
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503908 100 µg 228€
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Description

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant mouse IL-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1-5,8: The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody (Cat. No. 504002) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Abrams, J., et al. 1988. J. Immunol. 140:131.
  3. Cockayne, D., et al. 1991. Growth Factors 5:171.
  4. Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
  5. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  6. Finkelman, F., et al. 1993. J. Immunol. 151:1235.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Karulin, A., et al. 2000. J. Immunol. 164:1862.
Product Citations
  1. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2810627 (BioLegend Cat. No. 503907)
AB_2810628 (BioLegend Cat. No. 503908)

Antigen Details

Structure
Cytokine; 28 kD (Mammalian)
Bioactivity
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors
Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.

Regulation
Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID
16187 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-3 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-3 MP2-8F8 ICFC
Purified anti-mouse IL-3 MP2-8F8 ELISA Capture,IHC,IP
APC anti-mouse IL-3 MP2-8F8 ICFC
Ultra-LEAF™ Purified anti-mouse IL-3 MP2-8F8 ELISA Capture,ELISPOT Capture,Neut
Go To Top Version: 1    Revision Date: 05.10.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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