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Alexa Fluor® 594 anti-Cytoplasmic Dynein Intermediate Chain Antibody

Pricing & Availability
Clone
74.1 (See other available formats)
Regulatory Status
RUO
Other Names
Dynein light chain roadblock-type 1, Roadblock-1, ROBL/LC7-like 1, bithoraxoid-like protein, roadblock domain containing 1, dynein-associated protein Km23, cytoplasmic dynein light chain 2A, dynein light chain 2A, cytoplasmic
Isotype
Mouse IgG2b, κ
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Product Citations
publications
74dot1_A594_Cytoplasmic_Dynein_Int_Chain_Antibody_ICC_051019.png
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A-D) Alexa Fluor® 594 Anti- Cytoplasmic Dynein Intermediate Chain Antibody (Clone 74.1) or (E) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) overnight at 4°C. (B, D) Images are separate Alexa Fluor® 594 channel of images (A, C). Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time for all images (A-E) is 1/20 seconds. Concentrations for (A, B, E) is 5 µg/mL (1:100 dilution) and (C, D) is 2.5 µg/mL (1:200 dilution).
  • 74dot1_A594_Cytoplasmic_Dynein_Int_Chain_Antibody_ICC_051019.png
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A-D) Alexa Fluor® 594 Anti- Cytoplasmic Dynein Intermediate Chain Antibody (Clone 74.1) or (E) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) overnight at 4°C. (B, D) Images are separate Alexa Fluor® 594 channel of images (A, C). Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time for all images (A-E) is 1/20 seconds. Concentrations for (A, B, E) is 5 µg/mL (1:100 dilution) and (C, D) is 2.5 µg/mL (1:200 dilution).
See Alexa Fluor® 594 spectral data
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904903 25 µg 128€
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904904 100 µg 296€
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability.
Description

Dyneins are motor proteins that transport various cellular cargo along cytoskeletal microtubules in eukaryotic cells. Dynein has the same basic structures in all cell types and is composed of two or three distinct heavy chains (450 kD), three intermediate chains (70-125 kD), and at least four light chains (15-25 kD).  The cytoplasmic dynein intermediate chain is thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against purified bovine brain cytoplasmic dynein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunohistochemistry (IHC), immunoblotting and immunoprecipitation (IP).

*Predicted MW = 74 kD. This antibody is known not to react in squid and nematode.

Clone 74.1 reacts with all isoforms from both rat IC74 genes. The antigenic site is believed to be located in the conserved amino-terminal 60 amino acids. Clone 74.1 has been shown to inhibit the activity of cytoplasmic dynein.

Application References

(PubMed link indicates BioLegend citation)
  1. Miller MG, et al. 1999. Biol Reprod. 60:1047.
  2. Dillman JF, et al. 1996. Proc Natl Acad Sci USA. 93:141.
  3. Pfister KK, et al. 1996.J Biol Chem. 271:1687.
  4. Dillman JF, et al. 1994. J Cell Biol. 127:1671.
  5. Combs J, et al. 2006. PNAS. 103:14883.
  6. Chen JL, et al. 2005. J Cell Biol. 169:383. (IP) PubMed
  7. Burakov A, et al. 2008. Traffic. 9:472.
  8. Payne C, et al. 2003. J Cell Sci. 116:4727. (ICC) PubMed
  9. Semenova I, et al. 2014. Mol Biol Cell. 25:3119. (WB) PubMed
RRID
AB_2810813 (BioLegend Cat. No. 904903)
AB_2810814 (BioLegend Cat. No. 904904)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
83658 View all products for this Gene ID
UniProt
View information about Cytoplasmic Dynein Intermediate Chain on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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