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MojoSort™ Mouse anti-PE Nanobeads

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Product Citations
publications
MojoSort_Mouse_PE_Nanobeads_1_updated_012422
A single cell suspension from C57BL/6 mouse spleen was prepared and stained with NK-1.1 (clone PK136) PE. PE-positive cells were then isolated using MojoSort™ mouse anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_PE_Nanobeads_1_updated_012422
    A single cell suspension from C57BL/6 mouse spleen was prepared and stained with NK-1.1 (clone PK136) PE. PE-positive cells were then isolated using MojoSort™ mouse anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_PE_Nanobeads_2_updated_012422
    A single cell suspension from C57BL/6 mouse spleen was prepared and stained with CD4 (clone RM4-5) PE. PE-positive cells were then isolated using MojoSort™ Mouse Anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
Cat # Size Price Quantity Check Availability Save
480079 100 µL 138€
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480080 1 mL 408€
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Description

Mouse splenocytes or other single cell suspensions are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-mouse PE-conjugated antibody, followed by incubating with directly conjugated anti-PE Nanobeads. The PE+, magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the PE+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480079:

  • 1 vial containing 100 µl of Mouse anti-PE Nanobeads

For Cat# 480080:

  • 1 vial containing 1 ml of Mouse anti-PE Nanobeads

Product Details

Verified Reactivity
Mouse
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl PE Nanobeads for 1 X 107 cells in 100 µl of buffer.

Application Notes

The MojoSort™ Mouse anti-PE Nanobeads are designed to use with BioLegend's PE (phycoerythrin) conjugated antibodies against mouse cells. It can be used as a two-step kit for the positive selection or depletion of PE+ labeled cells from lymphoid or other organ tissues.

 

Product Citations
  1. Lin CH, et al. 2021. J Exp Med. 218:. PubMed
  2. Ma K, et al. 2022. iScience. 25:104347. PubMed

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 0    Revision Date: 04.14.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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