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Purified anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_WB_100608
Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
  • L5_WB_100608
    Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.
Compare all formats
Cat # Size Price Save
637319 25 µg ¥19,580
637301 200 µg ¥55,660
637302 500 µg ¥84,040
637303 1 mg ¥104,500
637304 5 mg ¥171,600
Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, ELISA, FC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. DaRosa PA et al. 2018. Molecular cell. 72(4):753-765 . PubMed
  2. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  3. Zhu C, et al. 2020. Cell Rep. 31:107745. PubMed
  4. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  5. Bala Tannan N, et al. 2018. PLoS Genet. 14:e1007153. PubMed
  6. Asano Y, et al. 2021. Nat Commun. 12:4372. PubMed
  7. Das A, et al. 2016. J Biol Chem. 291: 6096 - 6110. PubMed
  8. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  9. Chaouat AE, et al. 2021. PLoS Pathog. 17:e1010175. PubMed
  10. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
  11. Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed
  12. Wang X, et al. 2018. Pathog Dis. 76:. PubMed
  13. Sundaram A et al. 2017. eLife. 6 pii: e27187. PubMed
  14. Wen XY, et al. 2018. JCI Insight. 3:. PubMed
  15. Sánchez-Bellver L, et al. 2022. Int J Mol Sci. 23:. PubMed
  16. He H, et al. 2021. J Immunother Cancer. 9:. PubMed
  17. Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
  18. Bonet-Ponce L, et al. 2020. Sci Adv. 6:00. PubMed
  19. Ren S, et al. 2016. PLoS One. 11: 0152721. PubMed
  20. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  21. Nabar NR, et al. 2022. Autophagy. 18:204. PubMed
  22. Toyo-oka K, et al. 2014. J Neurosci. 34:12168. PubMed
  23. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
  24. Sønder S, et al. 2012. J Immunol. 188:5906. PubMed
  25. Golec E, et al. 2019. FASEB J. 33:12425. PubMed
  26. Velay F, et al. 2022. Plant Methods. 18:69. PubMed
  27. Adam S, et al. 2021. Nat Cancer. 2:1357. PubMed
  28. Yin J, et al. 2015. Brain. 138: 2553-2570. PubMed
  29. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  30. Karunakaran MM, et al. 2020. Immunity. 52:487. PubMed
  31. Moon S, et al. 2010. J Biol Chem. 285:12935. PubMed
  32. Yuan Y, et al. 2020. Nat Commun. 11:1472. PubMed
  33. Zang RX, et al. 2022. ACS Omega. 7:34378. PubMed
  34. Kim J, et al. 2021. J Cell Biol. 220:. PubMed
  35. Zhang S, et al. 2020. Nat Commun. 11:1312. PubMed
  36. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  37. Carlevaro G, et al. 2019. Methods Mol Biol. 1955:135. PubMed
  38. Okamoto Y, et al. 2021. J Cell Sci. 134:. PubMed
  39. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  40. Stroukov W, et al. 2019. Front Genet. 10:163. PubMed
  41. Cross SH, et al. 2020. PLoS Genet. 16:e1008583. PubMed
  42. Takahashi K, et al. 2020. Cell Reports. 31(9):107715. PubMed
  43. Liu X, et al. 2021. Hum Mol Genet. 30:30. PubMed
  44. Hu J, et al. 2021. Cell Death Dis. 12:391. PubMed
  45. Sandhu R, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  46. Xu C, et al. 2019. J Immunol. 203:2141. PubMed
  47. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  48. Liu X, et al. 2019. Nat Commun. 10:3020. PubMed
  49. Mamedov T, et al. 2019. Sci Rep. 9:9868. PubMed
  50. Driskill JH, et al. 2021. Genes Dev. 35:495. PubMed
  51. Yang Q, et al. 2020. Elife. 9:00. PubMed
  52. Torres-Ayuso P, et al. 2021. Cancer Discovery. 11(6):1411-1423. PubMed
  53. Figueroa F, et al. 2021. Front Cell Dev Biol. 9:739445. PubMed
  54. Sharma VP, et al. 2021. Nat Commun. 12:7300. PubMed
RRID
AB_2749907 (BioLegend Cat. No. 637319)
AB_1134266 (BioLegend Cat. No. 637301)
AB_1134268 (BioLegend Cat. No. 637302)
AB_1134265 (BioLegend Cat. No. 637303)
AB_1134267 (BioLegend Cat. No. 637304)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

I am unable to detect FLAG tag with L5 antibody clone by IP and/or western blotting.
Some points to consider here include
  • Sometimes changing tag location (form N-terminus to C-terminus or vice versa) or use tandem FLAG tag (3x) can also increase the chances of tag detection
  • Tagged protein is either not expressed or lowly expressed
  • Always include a positive control to make sure the process of working fine
Does anti tag antibody recognize the tag sequence at N terminal or C terminal or within the fusion protein?
Our antibody can recognize the fusion protein with tag either at N-terminus or C-terminus or within the protein if the tag is exposed.
Go To Top Version: 4    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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