Loading...

FITC anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
IM7
C57BL/6 splenocytes stained with IM7 FITC
  • IM7
    C57BL/6 splenocytes stained with IM7 FITC
  • IM7_FITC_060710
    Human peripheral blood lymphocytes stained with IM7 FITC
Compare all formats See FITC spectral data
Cat # Size Price Save
103021 25 tests ¥9,240
103005 50 µg ¥10,340
103006 500 µg ¥24,200
103022 100 tests ¥24,640
Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang S, et al. 2019. Int J Oncol. 54:966. PubMed
  2. Yang L, et al. 2022. Front Oncol. 12:862250. PubMed
  3. Ye ZW, et al. 2022. Cell Mol Immunol. 19:588. PubMed
  4. Tan L, et al. 2022. Aging Dis. 13:1562. PubMed
  5. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  6. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  7. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  8. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  9. Israelow B, et al. 2020. bioRxiv. . PubMed
  10. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  11. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  12. Wang D, et al. 2018. Immunity. 48:659. PubMed
  13. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  14. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  15. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  16. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  17. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  18. Dong L, et al. 2021. Cancer Cell. . PubMed
  19. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  20. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
  21. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  22. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  23. Diao L, et al. 2022. iScience. 25:105511. PubMed
  24. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  25. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  26. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  27. Bagheri V et al. 2018. Journal of cellular physiology. 233(10):7036-7046 . PubMed
  28. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  29. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  30. Yang L, et al. 2021. JCI Insight. 6:. PubMed
  31. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  32. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  33. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  34. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
  35. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  36. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  37. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  38. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  39. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  40. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  41. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  42. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  43. Murai T, et al. 2013. J Cell Sci. 126:3284. PubMed
  44. Zhang W, et al. 2021. Cell Prolif. 54:e13058. PubMed
  45. Wang Z, et al. 2022. Ann Transl Med. 10:544. PubMed
  46. Ebina-Shibuya R, et al. 2021. Elife. 10:. PubMed
  47. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  48. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  49. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  50. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  51. Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
  52. Amici SA, et al. 2021. Front Immunol. 12:695947. PubMed
  53. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  54. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  55. Park SG, et al. 2021. J Vet Sci. 22:e16. PubMed
  56. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  57. Xia H, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L926. PubMed
  58. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  59. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  60. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  61. Wan X, et al. 2018. Nature. 560:107. PubMed
  62. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  63. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  64. Wu X, et al. 2022. Cell Death Dis. 13:557. PubMed
  65. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  66. Ahmed MF, et al. 2019. Exp Ther Med. 17:4154. PubMed
  67. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  68. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  69. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  70. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  71. Liu ZZ, et al. 2021. Autophagy. 17:2766. PubMed
  72. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  73. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  74. Sim S, et al. 2016. PLoS One. 11: 0152485. PubMed
  75. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  76. Zhang S, et al. 2021. Stem Cells Int. 6616240:2021. PubMed
  77. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  78. Matossian MD, et al. 2019. BMC Cancer. 19:205. PubMed
  79. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  80. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  81. Liu Z, et al. 2016. Sci Rep. 6:34416. PubMed
  82. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  83. Kamijo S, et al. 2013. J Immunol. 190:4489. PubMed
  84. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  85. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  86. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  87. Geburek F, et al. 2017. Stem Cell Res Ther. 8:129. PubMed
  88. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  89. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  90. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  91. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  92. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  93. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  94. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  95. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  96. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  97. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  98. Witschen PM, et al. 2020. Cancers. 12(5):1325. PubMed
  99. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  100. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  101. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  102. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  103. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  104. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  105. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  106. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  107. Byrne K, et al. 2014. J Immunol. 192:1433. PubMed
  108. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  109. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  110. Shah D, et al. 2018. Clin Cancer Res. 24:4566. PubMed
  111. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  112. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  113. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  114. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  115. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  116. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  117. Yang L, et al. 2022. Proteomics. 22:e2200018. PubMed
  118. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  119. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  120. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  121. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  122. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
  123. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  124. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  125. Bi R, et al. 2020. Osteoarthritis Cartilage. 28:842. PubMed
  126. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  127. Zhao Y, et al. 2020. Br J Cancer. 1638:122. PubMed
RRID
AB_493684 (BioLegend Cat. No. 103021)
AB_312956 (BioLegend Cat. No. 103005)
AB_312957 (BioLegend Cat. No. 103006)
AB_493685 (BioLegend Cat. No. 103022)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 2    Revision Date: 09/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account