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Biotin anti-mouse IgMb Antibody

Pricing & Availability
Clone
AF6-78 (See other available formats)
Regulatory Status
RUO
Other Names
Igh-6b
Isotype
Mouse (BALB/c) IgG1, κ
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Product Citations
publications
AF6-78_Biotin_030209.jpg
C57BL/6 mouse splenocytes stained with biotinylated AF6-78, followed by Sav-PE
  • AF6-78_Biotin_030209.jpg
    C57BL/6 mouse splenocytes stained with biotinylated AF6-78, followed by Sav-PE
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Cat # Size Price Save
406204 500 µg ¥61,820
Description

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-Cb and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-Ce can be detected with this antibody.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
ELISA Detection - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.

Application References

(PubMed link indicates BioLegend citation)
  1. Stall AM, et al. 1984. J. Immunol. 132:787.
  2. Velardi A, et al. 1984. J. Immunol. 133:2098.
Product Citations
  1. Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
  2. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  3. Sundling C, et al. 2021. Immunity. 54(5):988-1001.e5. PubMed
  4. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
RRID
AB_315037 (BioLegend Cat. No. 406204)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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