Product: ELISA MAX™ Standard Set Mouse IL-6

Catalog No.: 431303

Militovic Vladimir, PostDoc University of Novi Sad

Mouse IL-6 ELISA MAX™ Standard
Overall:	Product Quality:	Ease of Use:
Estimation of IL-6 cytokine levels.

Experimental Design

Application:	ELISA
Cells used:	Lymphocytes
Brief Protocol:	Pre-working day (24h before working): Dilute your Capture antibody in the Coating buffer and add 100 microliter/well to the 96 well/plate. Working day: Wash plate and block it for 1 hour with Assay diluent. After the blocking time wash and add your samples and standards and incubate for 2h in RT (room temperature). After 2h of incubation wash again and add the diluted Detection antibody (incubate for 1h). Then wash again and add the enzyme (diluted in Assay diluent) and incub ate for 30 min., then wash again and add the TMB Substrate solution. In the end use the Stop solution. Read the plate in a monochromator plate reader.
Results Summary:	IL-6 cytokine levels.
Additional Notes:

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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