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PerCP/Cyanine5.5 anti-mouse α-GalCer:CD1d complex Antibody

Pricing & Availability
Clone
L363 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L363_PerCPCy55_alpha-GalCer-Cd1d_Antibody_101218
Murine CD1d transfected RMA-s cells were treated with (filled histogram) or without (open histogram) a-GalCer overnight, then stained with a-GalCer:CD1d complex (clone L363) PerCP/Cyanine5.5.
  • L363_PerCPCy55_alpha-GalCer-Cd1d_Antibody_101218
    Murine CD1d transfected RMA-s cells were treated with (filled histogram) or without (open histogram) a-GalCer overnight, then stained with a-GalCer:CD1d complex (clone L363) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
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140513 25 µg 141€
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140514 100 µg 315€
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Description

The L363 antibody reacts with α-GalCer:mouse CD1d complex. Glycolipid α-galactosylceramide (α-GalCer) is a potent activator of human and murine iNKT cells and is widely used as a model antigen for the studies of iNKT-mediated immunity. The glycolipid binds to CD1d and forms a complex which is subsequently recognized by iNKT cells, a subset of T cells expressing invariant TCR α chain Vα14, analogous to the peptide-MHC complex recognition by TCR α/ß T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
α-GalCer:mCD1d1 complexes coupled to Mycobacterium tuberculosis PPD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Surface and intracellular immunofluorescence2 and block a-GalCer:mCD1d1 complex induced iNKT activation1.

Application References
  1. Yu KO, et al. 2007. J. Immunol. Methods 323:11. (Block)
  2. Im JS, et al. 2009. Immunity 30:888. (IF)
RRID
AB_2783101 (BioLegend Cat. No. 140513)
AB_2783102 (BioLegend Cat. No. 140514)

Antigen Details

Distribution

NKT cells

Cell Type
B cells, Dendritic cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References

1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
5. Sullivan BA and Kronenberg M. 2005. J. Clin. Invest. 115:2328.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about alpha-GalCer:CD1d complex on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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