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MojoSort™ Human CD41 Nanobeads

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Regulatory Status
RUO
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A_MojoSort_Human_CD41_Nanobeads_1_071317
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate or deplete CD41+ platelets using MojoSort™ Human CD41 Nanobeads. Top plot: CD41+ platelets after isolation. Middle plot: CD41- PBMCs after isolation. Bottom plot: Cells before isolation. Cells were stained with CD42b (clone HIP1) APC. Dead cells were excluded by 7-AAD.
  • A_MojoSort_Human_CD41_Nanobeads_1_071317
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate or deplete CD41+ platelets using MojoSort™ Human CD41 Nanobeads. Top plot: CD41+ platelets after isolation. Middle plot: CD41- PBMCs after isolation. Bottom plot: Cells before isolation. Cells were stained with CD42b (clone HIP1) APC. Dead cells were excluded by 7-AAD.
  • B_MojoSort_Human_CD41_Nanobeads_2_071317
  • C_MojoSort_Human_CD41_Nanobeads_3_071317
Cat # Size Price Quantity Check Availability Save
480083 10 tests 228€
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480084 100 tests 661€
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Description

CD41+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD41 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD41+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480083:

  • 1 vial containing 100 µl of Human CD41 Nanobeads

For Cat# 480084:

  • 1 vial containing 1 ml of Human CD41 Nanobeads

Product Details

Verified Reactivity
Human
Formulation
Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated Nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of Human CD41 Nanobeads for 1 x 107 cells in 100 µl of buffer. If the frequency of CD41+ cells is lower than 50%, or red cells are lysed prior magentic isolation, the volume of Nanobeads to be used needs to be titrated down.

Application Notes

This kit is designed for the positive selection or depletion of human CD41+ cells from peripheral blood mononuclear cells (PBMCs).
 

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 8X

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD41 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

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Go To Top Version: 1    Revision Date: 07/14/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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