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Anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39200)

Pricing & Availability
Clone
4G8 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9200-02
Signet Catalog# 9200-05
Signet Catalog# 9200-10
Covance Catalog# SIG-39200
Isotype
Mouse IgG2b, κ
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Product Citations
publications
4G8_Ascites_Beta-Amyloid_Antibody_1_091118
IHC staining of anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with the primary antibody at 1:5000 dilution overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • 4G8_Ascites_Beta-Amyloid_Antibody_1_091118
    IHC staining of anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with the primary antibody at 1:5000 dilution overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • 4G8_Ascites_Beta-Amyloid_Antibody_2_091118
    IHC staining of anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with the primary antibody at 1:5000 dilution overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G8_Ascites_Beta-Amyloid_Antibody_3_091118
    Direct ELISA of anti-β-Amyloid, 17-24 (clone 4G8) antibody binding to the plate-immobilized human Aβ1-40, human Aβ1-42 and recombinant human APP751 protein. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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800708 50 µL 240€
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800709 200 µL 844€
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800710 500 µL 1715€
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800711 1 mL 2875€
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Description

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
Application

IHC-P - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Direct ELISA applications, a dilution range of 1:100 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms.

This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).

Application References
  1. Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed
  2. Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed
  3. Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875:87. (IHC-P) PubMed
  4. Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed
  5. Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed
Product Citations
  1. Klakotskaia D, et al. 2018. PLoS One. 8:8755. PubMed
  2. Choi S, et al. 2015. PLoS One. 10: 0143854. PubMed
  3. Woelfle S, et al. 2021. Front Cell Neurosci. 15:789903. PubMed
  4. Taddei RN, et al. 2022. Acta Neuropathol Commun. 10:72. PubMed
  5. Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed
  6. Martin L, et al. 2021. Cell Reports. 35(6):109121. PubMed
  7. Gkanatsiou E, et al. 2021. J Neurochem. 159:258. PubMed
  8. Jiwaji Z, et al. 2022. Nat Commun. 13:135. PubMed
  9. Morales R, et al. 2021. Mol Psychiatry. 26:5690. PubMed
  10. Whitehouse I, et al. 2016. PLoS One. 11: 0159119. PubMed
  11. Chaudry O, et al. 2022. Sci Rep. 12:19129. PubMed
  12. Robertson AS, et al. 2017. Neuromuscul Disord. 27:635. PubMed
  13. Mu L, et al. 2022. Int J Mol Sci. 23:. PubMed
  14. Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed
  15. Gamez N, et al. 2022. Cells. 11:. PubMed
  16. Schaefer P, et al. 2016. PLoS One. 11:e0168157. PubMed
  17. Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
  18. Lessard C, et al. 2015. PLoS One. 10: 0144758. PubMed
  19. Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed
  20. Zhang J, et al. 2021. iScience. 24:103327. PubMed
  21. Tang Z, et al. 2019. Nat Commun. 10:2173. PubMed
  22. Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed
  23. Maxwell AM, et al. 2021. Acta Neuropathol Commun. 9:201. PubMed
RRID
AB_2734547 (BioLegend Cat. No. 800708)
AB_2565325 (BioLegend Cat. No. 800709)
AB_2565326 (BioLegend Cat. No. 800710)
AB_2565324 (BioLegend Cat. No. 800711)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83.
  4. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595.
  6. Walsh DM, et al.  2007. J Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 17-24 on UniProt.org

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Go To Top Version: 4    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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