Product: ELISA MAX™ Deluxe Set Human GM-CSF

Catalog No.: 432004

Sojan Abraham, Post Doc TTUHSC

Very Good Human GMCSF ELISA Kit
Overall:	Product Quality:	Ease of Use:
Very useful for quantifying GMCSF in cell culture supernatants and serum.

Experimental Design

Application:	ELISA
Cells used:	Human GMCSF plasmid transfected cells
Brief Protocol:	1. Plates were coated with capture antibody overnight at 4 degree. 2. Plates were washed three times and blocked for 1 hr with 1X assay diluent. 3. Plates were washed three times and incubated with GMCSF standards and unknown samples for two hours. 4. Samples were washed three times and incubated with detection antibody for 1 hr. 5. Plates were washed three times and incubated with Avidin HRP for 30 min. 6. Plates were washed 4 times and incubated with 100 ul substrate solution for 15 minutes. 7. After 15 minutes 100 ul stop solution was added and absorbance was measured at 450.
Results Summary:	Able to quantify GMCSF concentration in cell culture supernatants.
Additional Notes:

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Publication Status This data has not been published.

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