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- Clone
- 1G5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Catenin (Cadherin-Associated Protein), Alpha 1, 102kD, Alpha-1-Catenin, Alpha E-Catenin , Cadherin-Associated Protein,102 kD, CAP102, CTNNA1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
MeOH fixed HeLa cells were stained with α-E-Catenin (clone 1G5) at 1 µg/ml. Goat-anti mouse Alexa Fluor® 594 was used as a secondary antibody and the cells were counterstained using DAPI. -
30 µg of A431 cell lysate was loaded and subjected to electrophoresis. Membrane was blotted using α-E-Catenin (clone 1G5) at 10 µg/ml followed by a fluorescent labeled goat anti-mouse IgG secondary.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 844801 | 25 µg | 90€ | ||||
| 844802 | 100 µg | 221€ | ||||
α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties. Catenin can associate with both E- and N-cadherins. It was originally thought to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and α-catenin was shown not to bind to F-actin when assembled in the complex suggesting a more complex, dynamic linkage between actin and these adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human MBP-alpha catenin fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
WB - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. - Application References
-
- Sacco PA, et al. 1995. J. Biol. Chem. 270:20201. (WB, IP, IF)
- Johnson KR, et al. 1993. Exp. Cell Res. 2:252.
- RRID
-
AB_2565821 (BioLegend Cat. No. 844801)
AB_2565808 (BioLegend Cat. No. 844802)
Antigen Details
- Structure
- A 102 kD member of the α-Catenin family composed primarily of alpha helical bundles. It contains three vinculin homology domains, a vinculin binding region, an actin binding domain, and a dimerization domain.
- Distribution
-
Cytoplasm, cytoskeleton, cell junctions, adherens junctions, cell membrane, peripheral membraine protein, and the cytoplasmic side.
- Function
- Involved in endothelial cell survival, migration, contact-dependent growth inhibition, hemophilic adhesion, signal transduction, Wnt signaling, and cell junction organization.
- Interaction
- α-catenin binds to β-catenin. Cadherin binds to the p120-catenin and β-catenin. This cadherin-catenin complex interacts with the actin cytoskeleton. α-catenin can also bind vinculin, then associate with actin nucleating proteins.
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
- Gene ID
- 1495 View all products for this Gene ID
- UniProt
- View information about alpha-E-Catenin on UniProt.org
Related FAQs
Other Formats
View All α-E-Catenin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-α-E-Catenin | 1G5 | ICC,WB,IP |
| Ultra-LEAF™ Purified anti-α-E-Catenin | 1G5 | ICC,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-α-E-Catenin
MeOH fixed HeLa cells were stained with α-E-Catenin (clone 1... 
30 µg of A431 cell lysate was loaded and subjected to electr... -
Ultra-LEAF™ Purified anti-α-E-Catenin
MeOH fixed HeLa cells were stained with α-E-Catenin (clone 1...
30 µg of A431 cell lysate was loaded and subjected to electr...
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