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Pacific Blue™ anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
  • 104_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
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109819 25 µg 76€
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109820 100 µg 172€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  2. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Israelow B, et al. 2020. bioRxiv. . PubMed
  5. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  6. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  7. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  8. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  9. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  10. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  11. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  12. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  13. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
  14. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  15. Yalamanoglu A, et al. 2021. Front Immunol. 627944:12. PubMed
  16. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  17. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  18. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  19. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  20. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
  21. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  22. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  23. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  24. Keller CW, et al. 2020. Autophagy. 1:. PubMed
  25. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  26. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  27. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  28. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  29. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  30. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  31. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
  32. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
  33. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  34. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  35. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  36. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  37. Flommersfeld S, et al. 2021. Immunity. :. PubMed
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  40. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  41. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  42. Goldman E, et al. 2021. Cells. 10:. PubMed
  43. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
  44. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
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  47. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
  48. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  49. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  50. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  51. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  52. Pfefferlé M, et al. 2021. Front Immunol. 12:680855. PubMed
  53. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  54. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  55. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  56. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  57. Ma K, et al. 2022. iScience. 25:104347. PubMed
  58. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
  59. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  60. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
  61. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  62. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  63. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  64. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  65. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  66. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  67. Tullett KM, et al. 2020. Elife. 9:00. PubMed
  68. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  69. Müller A, et al. 2012. Immunity. 37:147. PubMed
  70. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  71. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  72. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
  73. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
  74. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  75. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  76. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
  77. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  78. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  79. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  80. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  81. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  82. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  83. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed
  84. Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed
RRID
AB_492873 (BioLegend Cat. No. 109819)
AB_492872 (BioLegend Cat. No. 109820)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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