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Purified anti-PPP5 Antibody

Pricing & Availability
Clone
O95G5 (See other available formats)
Regulatory Status
RUO
Other Names
Protein Phosphatase 5 Catalytic Subunit, Serine/Threonine-Protein Phosphatase 5, Protein Phosphatase T, PP5
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
O95G5_PURE_PPP5_Antibody_1_061719
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PPP5 Antibody, clone O95G5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • O95G5_PURE_PPP5_Antibody_1_061719
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PPP5 Antibody, clone O95G5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • O95G5_PURE_PPP5_Antibody_2_061719
    Whole cell extracts (300 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified Mouse IgG1, κ Isotype Control Antibody (Cat. No. 400102) or Purified anti-PPP5 Antibody, clone O95G5. The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1.0 µg/mL (1:500 dilution) of O95G5. Lane M: Molecular Weight marker.
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622501 25 µg 81€
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622502 100 µg 203€
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Description

Protein phosphatase 5 catalytic subunit (PPP5) is a member of the phosphoprotein phosphatase family of enzymes and shows specificity towards phosphorylated serine and threonine residues. PPP5 displays broad substrate recognition and regulates diverse signal transduction cascades, including hormone and stress response pathways. PPP5 contains an autoinhibitory tetratricopeptide repeat (TPR) domain; PPP5 activity is augmented by Hsp90A, which releases TPR inhibition of the enzyme and also promotes the interaction of the PPP5 catalytic domain with client proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human PP5 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with two unidentified proteins of ~45 and 50 kD. This protein was also detected in O95G5 immunoprecipitates from HeLa extracts. We therefore do not recommend using this clone for co-immunoprecipitations.

RRID
AB_2810683 (BioLegend Cat. No. 622501)
AB_2810684 (BioLegend Cat. No. 622502)

Antigen Details

Structure
PPP5 is a 499 amino acid protein with a predicted molecular weight of 57 kD.
Distribution

Nucleus, cytosol, plasma membrane/ubiquitously expressed

Function
Phosphatase enzyme
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Wechsler T, et al. 2004. Proc Natl Acad Sci USA. 101:1247.
  2. Swingle MR, et al. 2004. J Biol Chem. 279:33992.
  3. Oberoji J, et al. 2016. Proc Natl Acad Sci USA. 113:9009.
Gene ID
5536 View all products for this Gene ID
UniProt
View information about PPP5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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