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APC/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_APCCy7_022309.jpg
Mouse thymic epithelial stromal cell line TE-71 stained with G8.8 APC/Cyanine7
  • G8dot8_APCCy7_022309.jpg
    Mouse thymic epithelial stromal cell line TE-71 stained with G8.8 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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118217 25 µg 118€
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118218 100 µg 278€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed
  2. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  3. Zwarycz B, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:1. PubMed
  4. Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed
  5. Karlsson J, et al. 2021. Adv Funct Mater. 31:. PubMed
  6. Dumont-Lagacé M, et al. 2014. J Immunol. 192:2219. PubMed
  7. Ruth JR, et al. 2021. Breast Cancer Res. 23:63. PubMed
  8. Zaid A, et al. 2014. Proc Natl Acad Sci U S A. 111:5307. PubMed
  9. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  10. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  11. Agarwal S, et al. 2015. Cell Rep. 13: 2147-2158. PubMed
  12. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  13. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  14. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  15. Xu Q, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  16. Kim M, et al. 2015. J Immunol. 194:4748. PubMed
  17. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  18. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  19. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  20. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  21. Morral C, et al. 2020. Cell Stem Cell. 26(6):845-861. PubMed
  22. Guo Q, et al. 2022. Nat Commun. 13:3837. PubMed
  23. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  24. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  25. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  26. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  27. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  28. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  29. Wells KL, et al. 2020. Elife. 9:. PubMed
  30. Mulholland D, et al. 2012. Cancer Res. 72:1878. PubMed
  31. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  32. Manzo N, et al. 2012. Am J Respir Cell Mol Biol. 47:349. PubMed
  33. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  34. Douchi D, et al. 2022. Cell Mol Gastroenterol Hepatol. 13:1317. PubMed
  35. St-Pierre C, et al. 2015. J Immunol. 195: 498 - 506. PubMed
  36. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  37. Di Campli MP, et al. 2020. Eur Respir J. . PubMed
  38. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  39. Ikonomou L, et al. 2020. Nat Commun. 0.899305556. PubMed
  40. Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed
  41. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  42. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  43. Dumont-Lagacé M, et al. 2015. Sci Rep. 5: 12895. PubMed
  44. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  45. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  46. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  47. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  48. Garibaldi B, et al. 2013. Am J Respir Cell Mol Biol. 48:35. PubMed
  49. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  50. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  51. Bretou M, et al. 2017. Sci Immunol. 2:16. PubMed
  52. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_1501158 (BioLegend Cat. No. 118217)
AB_2098648 (BioLegend Cat. No. 118218)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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