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HRP anti-AMIGO-1 Antibody

Pricing & Availability
Clone
L86A/37 (See other available formats)
Regulatory Status
RUO
Other Names
Amphoterin-induced protein 1, alivin-2, amphoterin-induced gene and ORF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_L86Aslash37_HRP_AMIGO-1_IHCP_Antibody_1_061518
IHC staining of HRP anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1_L86Aslash37_HRP_AMIGO-1_IHCP_Antibody_1_061518
    IHC staining of HRP anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_L86Aslash37_HRP_AMIGO-1_IHCP_Antibody_2_061518
    IHC staining of HRP anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_L86Aslash37_HRP_AMIGO-1_Antibody_WB_061518
    Western blot of HRP anti-AMIGO-1 antibody (clone L86A/37). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
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822103 25 µg 95€
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822104 100 µg 160€
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Description

Amphoterin-induced protein 1 (AMIGO-1) is a transmembrane protein containing six LRR domains and one Ig domain and is highly expressed in the mammalian nervous system. It promotes growth and fasciculation of neurites from cultured hippocampal neurons and may be involved in fasciculation as well as myelination of developing neural axons. AMIGO-1 may have a role in regeneration as well as neural plasticity in the adult nervous system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Verified
WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml.It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

The reactivity of HRP anti-AMIGO-1 antibody was verified in FFPE mouse and rat brain tissues.

Application References
  1. Mandikian D, et al. 2014. Comp Neurol. 522(15): 3555. (IHC-P)
RRID
AB_2734589 (BioLegend Cat. No. 822103)
AB_2734590 (BioLegend Cat. No. 822104)

Antigen Details

Structure
Amigo-1 has an apparent molecular weight ranging from 60-80 kD.
Distribution

Tissue distribution: Nervous system<br>Cellular distribution: Plasma membrane

Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Adhesion Molecules
Antigen References

1. Chen Y, et al. 2006. Brain Res Rev. 51(2):265.

Gene ID
57463 View all products for this Gene ID
UniProt
View information about AMIGO-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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