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Fixation Buffer

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Regulatory Status
RUO
Other Names
Fixative, Paraformaldehyde
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420801 100 mL 48€
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Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC
Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  2. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  3. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  4. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  5. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  6. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  7. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  8. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  9. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  10. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  11. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  12. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  13. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  14. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  15. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  16. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  17. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  18. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  19. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  20. Garton J, et al. 2021. Immunohorizons. 5:802. PubMed
  21. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  22. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  23. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  24. de Groot AE, et al. 2021. Prostate. 81:629. PubMed
  25. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  26. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  27. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  28. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  29. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  30. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  31. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  32. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  33. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  34. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  35. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  36. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  37. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  38. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  39. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  40. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  41. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  42. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  43. Bayer AL, et al. 2022. Cancers (Basel). 14:. PubMed
  44. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  45. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  46. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  47. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  48. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  49. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  50. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  51. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  52. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  53. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  54. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  55. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  56. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  57. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  58. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  59. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  60. Huang M, et al. 2022. Adv Sci (Weinh). 9:e2101267. PubMed
  61. Li FJ, et al. 2022. PLoS One. 17:e0277019. PubMed
  62. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  63. Takahashi K, et al. 2022. Cell Rep Methods. 2:100155. PubMed
  64. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  65. Rada M, et al. 2021. Commun Biol. 4:950. PubMed
  66. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  67. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  68. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  69. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  70. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  71. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  72. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  73. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  74. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  75. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  76. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  77. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  78. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  79. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  80. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  81. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  82. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  83. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  84. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  85. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  86. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  87. Hornigold K, et al. 2022. Front Immunol. 13:888415. PubMed
  88. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  89. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  90. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  91. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  92. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  93. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  94. Willis RA, et al. 2021. Curr Protoc. 1:e36. PubMed
  95. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  96. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  97. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  98. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  99. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  100. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  101. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  102. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  103. Ma K, et al. 2022. iScience. 25:104347. PubMed
  104. Constant DA, et al. 2022. Immunohorizons. 6:416. PubMed
  105. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  106. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  107. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  108. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  109. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  110. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  111. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  112. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  113. Bruno MEC, et al. 2022. Geroscience. 44:1761. PubMed
  114. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  115. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  116. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  117. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  118. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  119. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  120. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  121. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  122. Gerson-Gurwitz A, et al. 2021. Front Oncol. 11:766298. PubMed
  123. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  124. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  125. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  126. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  127. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  128. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  129. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  130. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  131. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  132. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  133. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  134. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  135. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  136. Kissel T, et al. 2022. Sci Adv. 8:eabm1759. PubMed
  137. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  138. Haunerdinger V, et al. 2021. Front Immunol. 12:740047. PubMed
  139. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  140. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  141. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  142. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  143. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  144. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  145. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  146. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  147. Wang X, et al. 2022. Elife. 11:. PubMed
  148. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  149. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  150. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  151. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  152. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  153. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  154. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  155. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  156. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  157. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  158. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed
  159. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  160. Yoshikawa AM, et al. 2021. Nat Commun. 12:7106. PubMed
  161. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  162. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

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Go To Top Version: 4    Revision Date: 07/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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