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Biotin anti-Alpha-II Spectrin Antibody

Pricing & Availability
Clone
D8B7 (See other available formats)
Regulatory Status
RUO
Other Names
Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
D8B7_Biotin_Alpha-II-Spectrin_Antibody_1_070819
Western blot of Biotin anti-Alpha-II-Spectrin antibody (clone D8B7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system. The lower band in lane 2 is spectrin breakdown product (SBDP).
  • D8B7_Biotin_Alpha-II-Spectrin_Antibody_1_070819
    Western blot of Biotin anti-Alpha-II-Spectrin antibody (clone D8B7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system. The lower band in lane 2 is spectrin breakdown product (SBDP).
  • D8B7_Biotin_Alpha-II-Spectrin_Antibody_2_070819
    IHC staining of Biotin anti-Alpha-II-Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_Biotin_Alpha-II-Spectrin_Antibody_3_070819
    IHC staining of Biotin anti-Alpha-II-Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_Biotin_Alpha-II-Spectrin_Antibody_4_070819
    IHC staining of Biotin anti-Alpha-II-Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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803209 25 µg 109€
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803210 100 µg 249€
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Description

The spectrins are a family of widely distributed cytoskeletal proteins which are involved in actin crosslinking. Spectrin α-chain heterodimerizes with tissue-specific β-chains. It is specifically expressed in nonerythrocytic cells. The protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the gene are the cause of early infantile epileptic encephalopathy. Fodrin, a spectrin that seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Reactivity to Drosophila was only verified with the purified format.

Application References

(PubMed link indicates BioLegend citation)
  1. Konieczny P, et al. 2008. J Cell Biol. 181:667-81
  2. Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
  3. Xu J, et al. 2000. J Cell Science. 113:3805-14
  4. De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
  5. Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
  6. Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
  7. Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
  8. Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
  9. Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed
RRID
AB_2814539 (BioLegend Cat. No. 803209)
AB_2814540 (BioLegend Cat. No. 803210)

Antigen Details

Structure
Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. Pike BR, et al. 2001. J Neurochem. 78(6):1297-306.
  2. Weiss ES, et al. 2009. Ann Thorac Surg. 88(2):543
Gene ID
6709 View all products for this Gene ID
UniProt
View information about Alpha-II Spectrin on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 07/08/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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